Androgenesis at Fytagoras: selecting the right courgette microspores

Selection of the right (stage) microspores is essential for the induction of microspore cell division in androgenesis to produce doubled haploid plants. In Fytagoras’ research into these stages sometimes results in beautiful images. The picture, taken with a confocal scanning laser microscope, shows a courgette (Cucurbita pepo) microspore stained with DiOC(6). The research into the right microspores for androgenesis contributes strongly to Fytagoras’ great track record in the development of DH plant production protocols for notoriously difficult species.   Fytagoras is always working on a number of new and recalcitrant species. Based on Fytagoras’ extensive experience in DH technology and successes in several recalcitrant crops like e.g. tomato, more and more seed companies approach Fytagoras to produce doubled haploid plants.